Yes — based on the files you shared, your design does look like a 3.3 V analog acoustic front-end feeding an ADC, and the PCB file currently shows:
What I confirmed from your PCB
Table
Setting
Current value seen
Board outline
100 mm x 100 mm
Trace width in routed segments
0.25 mm
Copper zone clearance on main copper zone
0.5 mm
So if you want to keep the board size exactly 100 mm x 100 mm, that is fine. You only need to make sure the design rules match your competition/manufacturing target.
How to set minimum trace width and clearance in KiCad
These settings are in the Board Setup window.
1) Open the PCB editor
Open your project in KiCad
Open the PCB Editor
2) Open Board Setup
Go to File -> Board Setup
In the left panel, open Design Rules
Then check these sections:
Constraints
Net Classes
3) Set the global minimum clearance
In Design Rules -> Constraints:
Find Minimum clearance
Set it to 0.25 mm
4) Set the minimum track width
In Design Rules -> Constraints:
Find Minimum track width
Set it to 0.25 mm
5) Set the default routing width
Still in Board Setup, go to Design Rules -> Net Classes
In the Default net class:
Set Track width to 0.25 mm
Set Clearance to 0.25 mm
If you use custom net classes, set those too
6) Save
Click OK
How to set copper zone clearance in KiCad
This is separate from normal track clearance.
If the zone already exists
In the PCB editor, click the copper zone outline or filled area
Press E to edit it, or right-click -> Properties
In the zone properties window:
Find Pad connections / Clearance or Clearance
Set it to 0.25 mm
Click OK
Refill zones by pressing B
If the zone inherits rules
Some KiCad versions let the zone use the net-class or board rule.
If there is an inheritance option, make sure it resolves to 0.25 mm
If you want zero ambiguity, set the zone clearance explicitly to 0.25 mm
What to check after changing it
After updating the rules:
Run DRC
In the PCB editor, click Inspect -> Design Rules Checker
Run the check
Look for:
clearance violations
unconnected items
silk over pads
edge clearance issues
Refill zones
Press B
Then run DRC again
Important note for your current board
From your file:
Trace width already appears to be 0.25 mm
The main copper zone appears to be 0.5 mm clearance
That means you likely only need to:
keep board size as-is
verify Board Setup minimums are 0.25 mm
edit the copper zone so its clearance is also 0.25 mm
refill zones
rerun DRC
Recommended final values
Table
Rule
Set to
Minimum track width
0.25 mm
Minimum clearance
0.25 mm
Default net class track width
0.25 mm
Default net class clearance
0.25 mm
Copper zone clearance
0.25 mm
For JLCPCB acceptance
A 100 mm x 100 mm board is usually okay if that exact size is intentional. I would not change it if your competition requires it. Just make sure:
Wirelessly connects nets on schematic. Used to organize schematics and separate functional blocks. To wirelessly connect net portals, give them same designator. #portal
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